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Related Experiment Videos

Anti-mutagenic compounds from corn.

A Burgos-Hernández1, R López-García, H Njapau

  • 1Department of Food Science, Louisiana State University Agricultural Center, Baton Rouge 70803-4200, USA. aburgos@guayacan.uson.mx

Food Additives and Contaminants
|September 13, 2001
PubMed
Summary

Corn extracts contain anti-aflatoxin factors that reduce AFB1 mutagenicity in Ames tests. These identified factors in corn fractions demonstrate significant antimutagenic activity against aflatoxin B1.

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Area of Science:

  • Food Science
  • Toxicology
  • Biochemistry

Background:

  • Aflatoxin B1 (AFB1) is a potent mutagenic mycotoxin found in contaminated food sources like corn.
  • Previous studies indicated uncharacteristic responses to AFB1 in the Salmonella/microsomal mutagenicity assay (Ames test) with corn, suggesting the presence of anti-aflatoxin factors.

Purpose of the Study:

  • To isolate and identify anti-aflatoxin factors in corn extracts.
  • To evaluate the antimutagenic activity of corn fractions against AFB1 using the Ames test.

Main Methods:

  • Corn was extracted and fractionated using thin-layer chromatography (TLC) with various solvent systems.
  • The Ames test, utilizing Salmonella tester strains TA98 and TA100 with metabolic activation (S9), was employed to assess mutagenicity and antimutagenic activity.

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  • Fractions were analyzed for toxicity to tester strains.

    • Main Results:

    • Several corn fractions exhibited dose-dependent antimutagenic activity against pure AFB1.
    • The tested corn extracts were non-toxic to the Salmonella tester strains.
    • TLC fractions demonstrating the most potent antimutagenic effects were selected for further characterization.

    Conclusions:

    • Corn contains factors that can inhibit the mutagenicity of aflatoxin B1.
    • These findings support the presence of natural antimutagens in corn that can mitigate AFB1 toxicity.