Screening antimutagenic and antiproliferative properties of extracts isolated from Jackfruit pulp (Artocarpus heterophyllus Lam)

Area of Science:

• Phytochemistry
• Pharmacology
• Toxicology

Background:

• Natural products are increasingly investigated for therapeutic potential.
• Jackfruit (Artocarpus heterophyllus Lam) is a tropical fruit with a history of traditional use.
• Understanding the bioactive compounds in jackfruit is crucial for its medicinal applications.

Purpose of the Study:

• To evaluate the antimutagenic and antiproliferative potential of jackfruit pulp extract.
• To identify fractions with significant chemoprotective properties.
• To investigate the effects against aflatoxin B1-induced mutagenicity and cancer cell growth.

Main Methods:

• Extraction and sequential fractionation of jackfruit pulp using RP-HPLC.
• Antimutagenicity testing using Salmonella typhimurium strains TA98 and TA100 with metabolic activation (S9).
• Antiproliferative assays on the M12.C3.F6 murine B-cell lymphoma cell line.

Main Results:

• Jackfruit pulp extract significantly reduced AFB1-induced mutagenicity.
• The extract demonstrated dose-dependent inhibition of M12.C3.F6 cancer cell proliferation.
• RP-HPLC fractionation yielded specific fractions with notable antimutagenic and/or antiproliferative activities.

Conclusions:

• Jackfruit pulp contains compounds with potent chemoprotective properties.
• These compounds can mitigate the mutagenic effects of aflatoxin B1.
• Jackfruit extract shows potential for inhibiting cancer cell line proliferation.