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A Behavioral Assay for Mechanosensation of MARCM-based Clones in Drosophila melanogaster
Published on: December 30, 2015
A heat-shock-activated cDNA encoding GAGA factor rescues some lethal mutations in the Drosophila melanogaster
H Granok1, B A Leibovitch, S C Elgin
1Department of Biology, Campus Box 1229, Washington University, St Louis, MO 63130, USA.
Abstract:
GAGA factor is an important chromosomal protein involved in establishing specific nucleosome arrays and in regulating gene transcription in Drosophila melanogaster. We developed a transgenic system for controlled heat-shock-dependent overexpression of the GAGA factor 519 amino acid isoform (GAGA-519) in vivo. Efficient production of stable protein from these transgenes provided genetic rescue of a hypomorphic Trithorax-like (Trl) lethal allele to adulthood. Nevertheless, supplemental GAGA-519 did not suppress position effect variegation (PEV), a phenomenon commonly used to measure dosage effects of chromosomal proteins, nor did it rescue other lethal alleles of Trl. The results suggest requirements for the additional isoforms of GAGA factor, of for more precise regulation of synthesis, to carry out the diverse functions of this protein.
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