Related Experiment Videos
Study of normal values in coagulation profile
K Pathepchotiwong1, P Tantiniti, C Sutthithampanich
1Department of Clinical Microscopy, Faculty of Medical Technology, Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Journal of the Medical Association of Thailand = Chotmaihet Thangphaet
|September 15, 2001
Summary
This study establishes normal hemostasis parameter values in healthy volunteers using the Behring Coagulation Timer (BCT). Fresh plasma yielded different results than lyophilized plasma for key coagulation factors and clotting times.
Area of Science:
- Clinical Biochemistry
- Hematology
- Diagnostic Hemostasis
Background:
- Establishing reliable reference ranges for hemostasis parameters is crucial for accurate clinical diagnosis.
- Variations in plasma processing, such as fresh versus lyophilized, can impact coagulation test results.
Purpose of the Study:
- To determine normal values for key hemostasis parameters in a healthy volunteer cohort.
- To compare hemostasis parameters between fresh and lyophilized plasma samples.
- To validate the use of the Behring Coagulation Timer (BCT) for routine hemostasis testing.
Main Methods:
- Utilized the Behring Coagulation Timer (BCT) for hemostasis analysis.
- Collected and processed plasma from healthy volunteers into fresh and lyophilized aliquots.
- Measured prothrombin time (PT), activated partial thromboplastin time (APTT), and coagulation factors I, II, VII, VIII, and IX.
Main Results:
- Established normal ranges (p5-p95) for PT (10.8-13.3 sec), APTT (31.4-48.0 sec), and coagulation factors I (1.82-4.65 g/l), II (64.83-96.5%), VII (46.88-113.5%), VIII (52.44-127.61%), and IX (67.87-116.94%).
- Significant statistical differences (p < 0.05) were observed in PT and APTT between fresh and lyophilized plasma.
- Coagulation factors I, II, VIII, and IX showed significantly higher levels in fresh plasma compared to lyophilized plasma.
Conclusions:
- The study provides essential reference ranges for hemostasis parameters in healthy individuals using the BCT.
- Plasma sample type (fresh vs. lyophilized) significantly influences hemostasis test results, necessitating careful consideration in clinical practice.
- These findings support the BCT as a reliable instrument for hemostasis testing while highlighting the importance of sample integrity.