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Standardization of platelet aggregation test in normal Thai adults
T Surapisitchat1, N Archararit, K Pathepchotiwong
1Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
Summary
This study determined optimal concentrations for platelet aggregation tests in Thai adults using adenosine diphosphate (ADP), adrenaline, and collagen. These concentrations are suitable for further research on platelet function in this population.
Area of Science:
- Hematology
- Clinical Biochemistry
Background:
- Platelet aggregation is crucial for hemostasis.
- Standardized platelet function tests are needed for diverse populations.
Purpose of the Study:
- To establish optimal concentrations of aggregating agents for platelet aggregation testing in normal Thai adults.
- To characterize platelet aggregation responses in a Thai cohort.
Main Methods:
- Turbidimetric method used to assess platelet aggregation.
- Tested aggregating agents: adenosine diphosphate (ADP), adrenaline, and collagen.
- Evaluated 52 healthy Thai adults (24 males, 28 females; ages 20-50).
Main Results:
- Optimal threshold concentrations: ADP (5-10 µM), adrenaline (2.5-10 µM), collagen (0.14-0.28 mg/mL).
- Collagen induced aggregation with a lag phase within 2 minutes.
- Biphasic aggregation curves observed in 53-65% with ADP and 81-87% with adrenaline.
Conclusions:
- Established reference ranges for platelet aggregation in Thai adults.
- Identified suitable concentrations of ADP, adrenaline, and collagen for future studies.
- Findings support the use of these agents and concentrations for platelet function assessment in this demographic.