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Updated: Oct 4, 2026

Coupled Assays for Monitoring Protein Refolding in Saccharomyces cerevisiae
Published on: July 9, 2013
[Heat shock-induced changes in the respiration of the yeast Saccharomyces cerevisiae]
E G Rikhvanov1, N N Varakina, T M Rusaleva
1Siberian Institute of Physiology and Biochemistry of Plants, Siberian Division, Russian Academy of Sciences, Irkutsk, Russia. eugene@sifibir.irk.ru
Abstract:
The incubation of Saccharomyces cerevisiae at elevated temperature (45 degrees C) stimulated the respiration of yeast cells and decreased their survival rate. The respiration-deficient mutant of this yeast was found to be more tolerant to the elevated temperature than the wild-type strain. At the same time, the cultivation of the wild-type strain in an ethanol-containing medium enhanced the respiration, catalase activity, and thermotolerance of yeast cells, as compared with their growth in a glucose-containing medium. It is suggested that the enhanced respiration of yeast cells at 45 degrees C leads to an intense accumulation of reactive oxygen species, which may be one of the reasons for the heat shock-induced cell death.
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