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Biosurfactant-rhamnolipid effects on yeast cells
E Vasileva-Tonkova1, D Galabova, E Karpenko
1Department of Microbial Biochemistry and Biosynthesis, Institute of Microbiology, Bulgarian Academy of Sciences, Acad. G. Bonchev str., bl. 26, 1113 Sofia, Bulgaria.
Letters in Applied Microbiology
|September 18, 2001
Summary
A novel biosurfactant (PS) from Pseudomonas sp. S-17 shows mild effects on Saccharomyces cerevisiae yeast cells, similar to Triton X-100. This suggests PS is ecologically safe for developing green cleaning technologies.
Area of Science:
- Microbiology
- Biochemistry
Background:
- Surfactants are widely used in various industries.
- Pseudomonas sp. S-17 produces a novel biosurfactant (PS).
- Triton X-100 is a well-known synthetic surfactant.
Purpose of the Study:
- To investigate the effect of novel biosurfactant PS on Saccharomyces cerevisiae 83-20 yeast cells.
- To compare the effects of PS with Triton X-100.
Main Methods:
- Investigated surfactant effects on yeast during growth and on separated cells.
- Assessed cell-permeabilizing effects by monitoring protein and enzyme release.
- Analyzed changes in polypeptide profiles of cells and membrane proteins.
Main Results:
- Biosurfactant PS did not alter yeast culture growth kinetics.
- PS altered polypeptide profiles of cells and membrane proteins similarly to Triton X-100.
- Both PS and Triton X-100 exhibited surface-located action without affecting intracellular yeast structures.
Conclusions:
- Biosurfactant PS and Triton X-100 have comparable surface-acting mechanisms on yeast cells.
- PS demonstrates mild effects on yeast, indicating potential ecological safety.
- PS holds promise for developing environmentally friendly and effective cleaning technologies.
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