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Differential transcription factor expression in human mononuclear cells in response to amphotericin B: identification
J D Cleary1, P D Rogers, S W Chapman
1Department of Clinical Pharmacy Practice, University of Mississippi School of Pharmacy, Jackson 39216, USA. Jcleary@medicine.umsmed.edu
Pharmacotherapy
|September 19, 2001
Summary
Amphotericin B alters gene expression in human monocytic cells. This antifungal drug affects genes involved in immune response, signal transduction, and cell differentiation, offering insights into its efficacy and toxicity.
Area of Science:
- Molecular biology
- Immunology
- Pharmacology
Background:
- Amphotericin B is a crucial antifungal agent.
- Understanding its cellular mechanisms is vital for optimizing treatment.
- Gene expression profiling provides insights into drug effects.
Purpose of the Study:
- To identify genes differentially expressed in human monocytic cells upon exposure to amphotericin B.
- To investigate the impact of amphotericin B on gene transcription in vitro.
Main Methods:
- Human monocytic cells (THP-1) were exposed to amphotericin B or media control.
- Total RNA was isolated, reverse transcribed to cDNA, and analyzed using human cDNA microarray technology.
- Gene expression changes were quantified by measuring probe hybridization differences.
Main Results:
- Amphotericin B exposure resulted in the upregulation of 16 transcripts and downregulation of 4 transcripts out of 588 genes analyzed.
- Significant alterations in gene expression were observed, affecting cellular functions like immune response, signal transduction, and differentiation.
- These changes highlight the broad impact of amphotericin B on monocytic cell biology.
Conclusions:
- Amphotericin B demonstrably induces alterations in human cell gene transcription.
- The observed gene expression changes may serve as biomarkers for evaluating amphotericin B toxicity and efficacy in patients.
- Further research can leverage these findings to personalize antifungal therapy.