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Updated: Aug 17, 2026

The Use of Chemostats in Microbial Systems Biology
Published on: October 14, 2013
Growth dynamics of Agrobacterium tumefaciens in chemostat cultures limited by carbon source and mineral nutrients
Abstract:
Agrobacterium tumefaciens was grown in a chemostat in a chemically-defined medium which hs alpha-methyl D-glucoside, magnesium, manganese, phosphate or urea as the growth-limiting nutrient. Steady-state biomass concentrations were dependent on the specific growth rate of the organism when alpha-methyl D-glucoside, manganese or phosphate were growth-limiting nutrients. During magnesium-limited growth, large undamped oscillations in biomass concentration occurred. In all chemostat cultures a variant organism was selected which had lost the ability to grow in the medium supplied, bur survived on products of carbon metabolism derived from the wild-type.
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