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Updated: Oct 4, 2026

Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry
Published on: October 25, 2019
[Determination of clofelin in blood by gas chromatography-mass spectrometry]
Abstract:
Gas chromatography--mass spectrometry is proposed for measuring clofelin (clonidine) in cadaveric blood. The method includes liquid-liquid extraction of clonidine from the blood, derivatization with pentafluorobenzyl bromide, and subsequent purification of derivatization products before chromatographic analysis. The range of 0.5-50.0 ng/ml covers therapeutic and lethal concentrations of clonidine in the blood. The method was tried on expert material in Chelyabinsk Regional Bureau of Forensic Medical Expert Evaluations and can be used in forensic chemical and toxicological analysis.
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