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Updated: Jul 18, 2026

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
Published on: August 6, 2014
New approaches towards fluorescence labelling of messenger RNA transcripts
Abstract:
Fluorescence labelling of nucleic acids is being used for a wide range of biological applications. The performance of these techniques is dependent on fluorochrome labels with a high sensitivity and high resistance to photobleaching. Indo-cyanine dyes such as Cy3, Cy5 or Cy7 have been found to fulfill these requirements. This study describes several different RNA labelling techniques allowing for a Cy5 based detection of mRNA transcripts.
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