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Syntheses of DNA duplexes containing a C-C interstrand cross-link
A M Noronha1, D M Noll, P S Miller
1Johns Hopkins University, Department of Biochemistry and Molecular Biology, School of Hygiene and Public Health, 615 North Wolfe Street, Baltimore, Maryland, USA.
Nucleosides, Nucleotides & Nucleic Acids
|September 21, 2001
Abstract:
Short DNA duplexes that contain a N4C-ethyl-N4C interstrand cross-link were prepared on controlled pore glass supports using a DNA synthesizer. The C-C cross-link was introduced via a convertible nucleoside on the support or by using a protected C-C cross-link phosphoramidite. An orthogonal protection scheme allowed selective chain growth in either a 3'-->5' or 5'-->3' direction. The cross-linked duplexes were purified by HPLC and characterized by MALDI-TOF mass spectrometry and/or by enzymatic digestion.