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[Identification of notoginseng(Panax notoginseng) and its adulterants using DNA sequencing]
1Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700.
Objective:
To analyze the nuclear ribosomal RNA small subunit (18S rRNA) and chloroplast matK gene sequence of notoginseng (Panax notoginseng) in order to provide molecular evidence for its genuine origin identification.
Methods:
To sequence 18S rRNA and matK genes of Panax notoginseng and its four adulterants such as P. japonicus, Curcuma phaeocaulis, C. wenyujin, C. kwangsiensis using PCR direct sequencing and to detect their variation of sequences.
Results:
The sequence length of notoginseng and its adulterants is 1809-1811 bp for 18S rRNA gene and 1259-1548 bp for matK gene, respectively. Multiple sequence alignment shows that there are much sequence variation between notoginseng and its adulterants.
Conclusion:
DNA sequencing is an accurate and reliable method in origin identification of the genuine notoginseng.
