Related Experiment Video

Updated: Feb 5, 2026

A Pipeline to Investigate the Structures and Signaling Pathways of Sphingosine 1-Phosphate Receptors
12:27

A Pipeline to Investigate the Structures and Signaling Pathways of Sphingosine 1-Phosphate Receptors

Published on: June 8, 2022

4.0K

[Sodium Aescinate Induced Apoptosis of BGC-823 and AGS Cells by Inhibiting JAK-1/STAT-1 Signaling Pathway]

    Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials
    |September 14, 2018
    PubMed
    Abstract

    Insights

    Sodium aescinate inhibits gastric cancer cell proliferation and induces apoptosis by affecting cell morphology and nuclear changes. This occurs through the inhibition of the JAK-1/STAT-1 signaling pathway.

    Area of Science:

    • Oncology
    • Molecular Biology
    • Pharmacology

    Background:

    • Gastric cancer remains a significant global health challenge.
    • Understanding novel therapeutic mechanisms is crucial for effective treatment strategies.

    Purpose of the Study:

    • To elucidate the mechanism by which sodium aescinate induces apoptosis in gastric cancer cells.
    • To investigate the role of the JAK-1/STAT-1 signaling pathway in sodium aescinate's anti-cancer effects.

    Main Methods:

    • Cell viability was assessed using CCK-8 assays.
    • Apoptosis, cell morphology, and nuclear changes were evaluated via flow cytometry and DAPI staining.
    • Western blotting and laser confocal microscopy were employed to analyze JAK-1/STAT-1 phosphorylation and nuclear translocation.

    Main Results:

    • Sodium aescinate demonstrated dose-dependent inhibition of gastric cancer cell proliferation.
    • Significant induction of apoptosis and alterations in cell/nuclear morphology were observed.
    • Downregulation of JAK-1 and STAT-1 phosphorylation and inhibition of STAT-1 nuclear translocation were noted.

    Conclusions:

    • Sodium aescinate effectively inhibits proliferation and induces apoptosis in gastric cancer cells (BGC-823 and AGS).
    • The anti-cancer effects are mediated through the inhibition of the JAK-1/STAT-1 signaling pathway.

    More Related Videos

    Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway07:15

    Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway

    Published on: August 23, 2024

    950
    Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers11:45

    Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers

    Published on: March 8, 2012

    12.7K

    Related Concept Videos

    The JAK-STAT Signaling Pathway01:20

    The JAK-STAT Signaling Pathway

    Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
    12.6K
    Interpreting ¹H NMR Signal Splitting: The (n + 1) Rule01:10

    Interpreting ¹H NMR Signal Splitting: The (n + 1) Rule

    In the AX proton spin system, proton A can sense the two spin states of a coupled proton X, resulting in a doublet NMR signal with two peaks of equal (1:1) intensity. When proton A is coupled to two equivalent protons (AX2 spin system), the spin states of each X can be aligned with or against the external field, creating three possible scenarios. This results in a 1:2:1  triplet signal, where the central peak corresponds to the chemical shift of A and is twice as large or intense as the...
    2.7K
    SN1 Reaction: Stereochemistry02:15

    SN1 Reaction: Stereochemistry

    This lesson provides an in-depth discussion of the stereochemical outcomes in an SN1 reaction.
    In the first step of an SN1 reaction, the bond between the electrophilic carbon and the leaving group ionizes to generate the carbocation intermediate. The second step of the mechanism is the nucleophilic attack.
    In the formed carbocation, the positively charged carbon is sp2 hybridized with a trigonal planar geometry. As all the three substituents lie on the same plane, a plane of symmetry for the...
    10.4K
    SN1 Reaction: Kinetics02:05

    SN1 Reaction: Kinetics

    In an SN2 reaction, the reaction rate depends on both the type of nucleophile and the substrate. A hindered tertiary alkyl halide is practically inert to the SN2 mechanism despite using a strong nucleophile.
    However, Sir Christopher Ingold and Edward D. Hughes, who studied the kinetics of various nucleophilic substitution reactions, noticed that a tertiary alkyl halide does undergo a nucleophilic substitution reaction in the presence of a weak nucleophile. While studying the substitution...
    9.6K
    SN1 Reaction: Mechanism02:25

    SN1 Reaction: Mechanism

    Kinetic studies of ionization of a tertiary halide in a protic solvent suggest that only the substrate participates in the rate-determining step (slow step). The nucleophile is involved only after the slowest step. The SN1 reaction takes place in a multiple-step mechanism. 
    Firstly, the haloalkane ionizes to generate a carbocation intermediate and a halide ion. This heterolytic cleavage is highly endothermic with large activation energy. The ionization of the substrate, facilitated by a...
    14.3K
    Acidity of 1-Alkynes02:42

    Acidity of 1-Alkynes


    The acidic strength of hydrocarbons follows the order: Alkynes > Alkenes > Alkanes. The strength of an acid is commonly expressed in units of pKa — the lower the pKa, the stronger the acid. Among the hydrocarbons, terminal alkynes have lower pKa values and are, therefore, more acidic. For example, the pKa values for ethane, ethene, and acetylene are 51, 44, and 25, respectively, as shown here.
    11.2K