[Comparative Study on the Four Kinds of Flavonoids from Four Different Varieties of Dendranthema morifolium]

To establish a high phase liquid chromatography method of the content in quercetin,luteolin,apigenin,acacetin,and to compare the difference of content from four different varieties of Dendranthema morifolium in simultaneously.

The UPLC methods were adopted,and the chromatographic column was Waters ACQUITYUPLC; the column was BEH C18（ 50 mm ×2. 1 mm,1. 7 μm）,the mobile phase was 0. 1% phosphoric acid solution-methanol in gradient elution,the flow rate was 0. 2 m L/min;and the detection wavelength was set at 320 nm; the column temperature 25 ℃; and the sample quantity was 1 μL.

In the range of 0. 0027 0. 0135 mg/m L（ r1= 0. 9962） concentration within quercetin in a good linear relationship between peak area. In the range of0. 0032 0. 0160 mg/m L（ r2= 0. 9963） concentration within luteolin in a good linear relationship between peak area. In the range of0. 0029 0. 0145 mg/m L（ r3= 0. 9964） of apigenin in the mass concentration and the peak area. In the range of 0. 0029 0. 0145 mg/m L（ r4= 0. 9963） concentration within acacetin in a good linear relationship between peak area.

This method can be determined daisy quercetin,luteolin,apigenin,acacetin content in Dendranthema morifolium.