Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Net-targeted mutant mice develop a vascular phenotype and up-regulate egr-1.

A Ayadi1, H Zheng, P Sobieszczuk

  • 1Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP, 1 Rue Laurent Fries, BP 163, 67404 Illkirch cedex, France.

The EMBO Journal
|September 22, 2001
PubMed
Summary

The study reveals Net

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lung cancer segmentation Using the Att-U-Net Model on PET-CT Images.

Radiography (London, England : 1995)·2026
Same author

Genome Wide Conditional Mouse Knockout Resources.

Drug discovery today. Disease models·2024
Same author

2022 TUNISIAN NATIONAL CONGRESS OF MEDICINE ABSTRACTS.

La Tunisie medicale·2023
Same author

Investigations of the charge transfer phenomenon at the hybrid dye/BiVO<sub>4</sub> interface under visible radiation.

RSC advances·2022
Same author

Long-term therapeutic outcomes of facial arteriovenous malformations: A retrospective bi-centric study of 30 cases.

Annales de chirurgie plastique et esthetique·2021
Same author

Implication of efflux pumps and <i>ERG11</i> genes in resistance of clinical <i>Trichosporon asahii</i> isolates to fluconazole.

Journal of medical microbiology·2021

Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Vascular Biology

Background:

  • Ternary complex factors (TCFs) like Net regulate immediate early genes in vitro.
  • The in vivo roles of TCFs, particularly Net, in vascular development remain largely unknown.
  • Net acts as a repressor and is expressed during mouse vasculogenesis.

Purpose of the Study:

  • To investigate the in vivo function of Net in vascular development.
  • To explore the role of Net in the regulation of immediate early genes, such as egr-1, in vivo.
  • To characterize the phenotype of mice with a hypomorphic mutation in Net.

Main Methods:

  • Generation of gene-targeted mice expressing a hypomorphic Net mutant (Net delta) lacking the Ets DNA-binding domain.
  • Phenotypic analysis of homozygous mutant mice, including assessment of vascular defects and gene expression.

Related Experiment Videos

  • Analysis of Net's interaction with the egr-1 promoter and its binding to SRE-5.

    • Main Results:

    • Homozygous Net delta mutant mice exhibit vascular defects, including lymphangiectasis and chylothorax, leading to perinatal death.
    • Mutant mice show up-regulation of the immediate early gene egr-1 in the heart and pulmonary arteries.
    • Net negatively regulates the egr-1 promoter and specifically binds to SRE-5, indicating a direct regulatory role.

    Conclusions:

    • Net plays a crucial role in mouse vascular biology and lymphatic development.
    • Net is an in vivo regulator of egr-1, a gene implicated in vascular pathologies.
    • Dysregulation of Net and egr-1 may contribute to vascular and lymphatic abnormalities.