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Related Experiment Video

Updated: Jul 11, 2026

Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding

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Published on: February 26, 2015

Neocortex patterning by the secreted signaling molecule FGF8.

T Fukuchi-Shimogori¹, E A Grove

¹Department of Neurobiology, Pharmacology and Physiology, University of Chicago, Chicago, IL 60637, USA.

Science (New York, N.Y.)

|September 22, 2001

Summary

Fibroblast growth factor 8 (FGF8) signaling regulates the development of the mammalian neocortical area map. Manipulating FGF8 levels or sources alters area boundaries and can cause duplications, revealing its role in specifying positional identity.

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Area of Science:

Neuroscience
Developmental Biology
Genetics

Background:

The mammalian neocortex is organized into distinct functional areas.
The molecular mechanisms driving the formation of this area map during neurogenesis remain largely unknown.

Purpose of the Study:

To investigate the role of Fibroblast Growth Factor 8 (FGF8) in the development of the neocortical area map.
To elucidate the molecular mechanisms underlying the specification of positional identity in the neocortex.

Main Methods:

Utilized electroporation-mediated gene transfer in mouse embryos.
Manipulated the endogenous anterior FGF8 signal by augmenting or reducing its levels.
Introduced an ectopic posterior source of FGF8.

Main Results:

Augmenting anterior FGF8 signal shifted neocortical area boundaries posteriorly.
Reducing anterior FGF8 signal shifted area boundaries anteriorly.
Ectopic posterior FGF8 source induced partial area duplications, evidenced by ectopic somatosensory barrel fields.

Conclusions:

FGF8 signaling, originating from the anterior telencephalon, is a key regulator of neocortical area map development.
FGF8 plays a critical role in specifying positional identity within the developing neocortex.