Enzymatic endpoint analysis of glucose with the hexokinase method and the Union Carbide fast centrifugal analyzer

1. We describe an enzymatic endpoint method for glucose using the hexokinase reaction and the Union Carbide fast centrifugal analyzer. The new method permits the direct spectrophotometric measurement of serum blanks, and eliminates the need for separate blank determinations or computer-assisted calculation of the sample blank absorbance values. 2. The direct automatic blanking is accomplished by measuring the absorbance of the test solution 2 seconds after the reactants have been mixed. Our studies show that no appreciable conversion of glucose occurs during this time interval. 3. The results of the new method agree well with the results from the conventional hexokinase assay. In addition, the method requires only 5 microliters of serum, and has a high sample throughput rate.