Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A rapid method to map mutations in Drosophila.

S G Martin1, K C Dobi, D St Johnston

  • 1Wellcome/CRC Institute, Tennis Court Road, Cambridge CB2 1QR, UK. ds139@mole.bio.cam.ac.uk

Genome Biology
|September 28, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Symmetry breaking in the female germline cyst.

Science (New York, N.Y.)·2021
Same author

Nuclear displacement and fluorescence recovery after photobleaching (FRAP) assays to study division site placement and cytokinesis in fission yeast.

Methods in cell biology·2017
Same author

Inflammatory breast cancer: time to standardise diagnosis assessment and management, and for the joining of forces to facilitate effective research.

British journal of cancer·2015
Same author

Analysis of lymphatic and blood vessel invasion biomarkers in T1 esophagogastric adenocarcinomas for improved patient prognostication.

Diseases of the esophagus : official journal of the International Society for Diseases of the Esophagus·2014
Same author

BJR radiobiology special feature.

The British journal of radiology·2014
Same author

Redox proteins and radiotherapy.

Clinical oncology (Royal College of Radiologists (Great Britain))·2014
Same journal

Somatic mobility of transposons is explosive and shaped by distinct integration biases in Arabidopsis thaliana.

Genome biology·2026
Same journal

UK Biobank whole-genome sequencing reveals robust contributions of rare variants to complex-trait heritability.

Genome biology·2026
Same journal

A one-week automated genome-wide optical pooled screen using OttoSeq.

Genome biology·2026
Same journal

Integrated lipidomic and transcriptomic profiling of the host response in human malaria.

Genome biology·2026
Same journal

Centromeric satellite expansion drives genome evolution in the snowy owl.

Genome biology·2026
Same journal

Mapping the landscape of allele-specific expression in porcine genomes.

Genome biology·2026
See all related articles

Researchers developed a single-nucleotide polymorphism (SNP) map for Drosophila chromosome 3R. This map accelerates the identification of mutated genes from genetic screens, reducing time and cost.

Area of Science:

  • Genetics and Genomics
  • Developmental Biology
  • Drosophila melanogaster Research

Background:

  • Genetic screens in Drosophila are crucial for understanding cellular and developmental processes.
  • Clonal screens using the flp/FRT system enable mutant phenotype analysis across various cell types and developmental stages.
  • Identifying mutated genes is a bottleneck due to limitations in traditional mapping strategies for linking genetic markers to molecular maps.

Purpose of the Study:

  • To develop a single-nucleotide polymorphism (SNP) map for Drosophila chromosome arm 3R.
  • To improve the efficiency and reduce the cost of mapping mutations identified through genetic screens.

Main Methods:

  • Development of a SNP map for chromosome arm 3R, containing 73 polymorphisms between standard and mapping chromosomes.

Related Experiment Videos

  • Utilized a mapping chromosome with visible markers (rucuca) at an average density of one SNP per 370 kilobases (kb).
  • Employed meiotic mapping crosses and SNP detection reactions to pinpoint mutant locations.

    • Main Results:

    • Successfully mapped mutants to a 400 kb interval using a few hundred SNP detection reactions in a single cross.
    • Further SNP discovery refined mapping to approximately 50 kb within the region of interest.
    • Demonstrated a significant increase in mapping resolution and efficiency compared to standard methods.

    Conclusions:

    • Combining visible markers with molecular polymorphisms in a unified mapping strategy drastically reduces time and cost.
    • This approach requires at least four times fewer SNP detection reactions than traditional methods.
    • The developed SNP map facilitates rapid identification of molecular lesions in mutants from clonal screens.