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Serotype-specific detection of coxsackievirus A16 in clinical specimens by reverse transcription-nested PCR

J W Bendig1, P S O'Brien, P Muir

  • 1PHLS Coxsackievirus Reference Unit, Epsom, Surrey, United Kingdom. justin.bendig@virgin.net

Journal of Clinical Microbiology
|September 28, 2001
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...