Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Endoplasmic Reticulum01:39

Endoplasmic Reticulum

Endoplasmic ReticulumThe endoplasmic reticulum (ER) is an extensive network of membranous sacs and tubules in eukaryotic cells, continuous with the outer membrane of the nucleus. This structural continuity integrates nuclear and cytoplasmic processes and facilitates efficient intracellular transport. This allows mRNA to move directly from the nucleus to ribosomes for efficient protein synthesis. As a result, the ER serves as a central site for the synthesis, processing, and distribution of...
Golgi Apparatus01:49

Golgi Apparatus

As they leave the Endoplasmic Reticulum (ER), properly folded and assembled proteins are selectively packaged into vesicles. These vesicles are transported by microtubule-based motor proteins and fuse together to form vesicular tubular clusters, subsequently arriving at the Golgi apparatus, a eukaryotic endomembrane organelle that often has a distinctive ribbon-like appearance.The Golgi apparatus is a major sorting and dispatch station for the products of the ER. Newly arriving vesicles enter...
Golgi Apparatus01:09

Golgi Apparatus

Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
The Endoplasmic Reticulum01:43

The Endoplasmic Reticulum

The endoplasmic reticulum or ER makes up for more than half of the membranes in a cell and accounts for 10% of total cell volume. It is also the primary protein and lipid synthesis factory for most cell organelles, such as the Golgi apparatus, lysosomes, secretory vesicles, and the plasma membrane. Despite being the most extensive and functionally complex subcellular organelle, ER was the last to be discovered. After years of deliberation, Keith Porter and George Palade in the year 1954,...
Smooth Endoplasmic Reticulum01:21

Smooth Endoplasmic Reticulum

Smooth endoplasmic reticulum or smooth ER is a sub-organelle with specialized functions in animal cells and plant cells. It is often associated with the tubule morphology of the endoplasmic reticulum.
The ER provides optimal conditions for synthesizing steroid hormones and lipids, such as phospholipids and triglycerides. Traditionally, lipid metabolism was considered to be a smooth ER function. However, there is no direct evidence to prove that rough ER is completely excluded from lipid...
The Endoplasmic Reticulum01:43

The Endoplasmic Reticulum

The endoplasmic reticulum or ER makes up for more than half of the membranes in a cell and accounts for 10% of total cell volume. It is also the primary protein and lipid synthesis factory for most cell organelles, such as the Golgi apparatus, lysosomes, secretory vesicles, and the plasma membrane. Despite being the most extensive and functionally complex subcellular organelle, ER was the last to be discovered. After years of deliberation, Keith Porter and George Palade in the year 1954,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Phencyclidine (PCP), in Nanomolar Concentrations, Binds to Synaptosomes and Blocks Certain Potassium Channels: Covalent Labeling of K Channels with PCP.

Biophysical journal·2009
Same author

Origin of axon membrane hyperpolarization under sucrose-gap.

Biophysical journal·2009
Same author

Directionality in drug action on sodium-calcium exchange.

Annals of the New York Academy of Sciences·2007
Same author

Na/Ca exchanger and PMCA localization in neurons and astrocytes: functional implications.

Annals of the New York Academy of Sciences·2002
Same author

Anorexic effect of K+ channel blockade in mesenteric arterial smooth muscle and intestinal epithelial cells.

Journal of applied physiology (Bethesda, Md. : 1985)·2001
Same author

Interaction of a toxin from the scorpion Tityus serrulatus with a cloned K+ channel from squid (sqKv1A).

Biochemistry·2001

Related Experiment Video

Updated: Jul 22, 2026

Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading (TED)
09:32

Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading (TED)

Published on: May 7, 2013

Structural complexity and functional diversity of endoplasmic reticulum Ca(2+) stores.

M P Blaustein1, V A Golovina

  • 1Department of Physiology, University of Maryland School of Medicine, 655 W. Baltimore Street, Baltimore, MD 21201, USA. mblauste@umaryland.edu

Trends in Neurosciences
|September 29, 2001
PubMed
Summary

Endoplasmic reticulum (ER) stores have distinct compartments for calcium (Ca2+) loading and unloading. This organization in neurons and glia generates complex Ca2+ signals controlling cell functions.

More Related Videos

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells
16:43

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells

Published on: February 18, 2014

Monitoring ER/SR Calcium Release with the Targeted Ca2+ Sensor CatchER+
12:30

Monitoring ER/SR Calcium Release with the Targeted Ca2+ Sensor CatchER+

Published on: May 19, 2017

Related Experiment Videos

Last Updated: Jul 22, 2026

Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading (TED)
09:32

Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading (TED)

Published on: May 7, 2013

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells
16:43

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells

Published on: February 18, 2014

Monitoring ER/SR Calcium Release with the Targeted Ca2+ Sensor CatchER+
12:30

Monitoring ER/SR Calcium Release with the Targeted Ca2+ Sensor CatchER+

Published on: May 19, 2017

Area of Science:

  • Cellular biology
  • Neuroscience
  • Calcium signaling

Background:

  • The endoplasmic reticulum (ER) is a key organelle for cellular calcium (Ca2+) homeostasis.
  • Evidence suggests ER Ca2+ stores are spatially compartmentalized, allowing differential loading and unloading.
  • Sub-plasmalemmal ER (jER) forms functional units with the plasma membrane (PL) called PLasmERosomes.

Purpose of the Study:

  • To investigate the functional significance of spatially-distinct ER Ca2+ stores.
  • To elucidate the role of PLasmERosomes in regulating intracellular ion concentrations.
  • To understand how ER organization contributes to complex Ca2+ signaling.

Main Methods:

  • Direct observation of cellular structures.
  • Analysis of protein clusters in PL and jER.
  • Investigating ion transport mechanisms.

Main Results:

  • ER Ca2+ stores comprise individually loadable and unloadable compartments.
  • PLasmERosomes integrate jER and PL microdomains.
  • Specific transport proteins in PL and jER regulate Na+ and Ca2+ in the cytosolic space.

Conclusions:

  • The compartmentalized ER structure, particularly PLasmERosomes, is crucial for generating diverse Ca2+ signals.
  • This organization enables simultaneous control of numerous neuronal and glial functions.
  • Understanding ER Ca2+ dynamics is vital for comprehending cellular communication.