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Related Experiment Videos

Chitinase in cucumber xylem sap.

S Masuda1, H Kamada, S Satoh

  • 1Noda Institute for Scientific Research, Chiba, Japan. susumu.masuda@nifty.ne.jp

Bioscience, Biotechnology, and Biochemistry
|October 2, 2001
PubMed
Summary

Cucumber roots secrete a chitinase enzyme into xylem sap. This acidic protein, a pathogenesis-related (PR) protein, is delivered to the plant's aboveground parts.

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Area of Science:

  • Plant biochemistry
  • Molecular biology
  • Plant pathology

Background:

  • Chitinases are enzymes that break down chitin, a major component of fungal cell walls.
  • Pathogenesis-related (PR) proteins are plant defense proteins induced during pathogen attack.
  • Xylem sap transports water and nutrients from roots to the rest of the plant.

Purpose of the Study:

  • To investigate the presence and characteristics of chitinase activity in cucumber xylem sap.
  • To identify and purify the specific chitinase protein secreted by cucumber roots.

Main Methods:

  • Collection and fractionation of xylem sap from cut cucumber stems.
  • Assay for chitinase activity in different fractions.
  • Protein purification using chromatographic techniques.
  • N-terminal amino acid sequencing of the purified protein.

Main Results:

  • Chitinase activity was detected in cucumber xylem sap.
  • A 28-kDa acidic protein with chitinase activity was purified.
  • The N-terminal amino acid sequence of the purified protein matched a known chitinase gene.
  • Evidence suggests cucumber roots produce and secrete this acidic chitinase into the xylem.

Conclusions:

  • Cucumber roots actively secrete an acidic chitinase into the xylem sap.
  • This secreted chitinase is likely a pathogenesis-related (PR) protein.
  • The enzyme is transported via xylem to cucumber's aboveground organs, potentially contributing to plant defense.

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