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A theoretical model of intracellular devitrification.

J O Karlsson1

  • 1Department of Mechanical Engineering, University of Illinois, Chicago, Illinois 60607, USA. karlsson@alum.mit.edu

Cryobiology
|October 2, 2001
PubMed
Summary
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Cell dehydration significantly impacts intracellular ice formation during warming after cryopreservation. A new model reveals how water transport affects devitrification, crucial for optimizing freeze-thaw protocols.

Area of Science:

  • Cryobiology
  • Biophysics
  • Cell Biology

Background:

  • Devitrification, or intracellular ice formation, during warming causes damage to cryopreserved cells.
  • Previous models of devitrification did not account for cell dehydration's influence on ice formation.
  • Understanding devitrification is critical for improving cell cryopreservation techniques.

Purpose of the Study:

  • To develop a new theoretical model for cell devitrification that incorporates cell dehydration.
  • To investigate the role of cell dehydration in intracellular ice formation during warming.
  • To explore how water transport kinetics affect nucleation and crystal growth during cryopreservation.

Main Methods:

  • Coupling membrane-limited water transport equations with classical nucleation theory and diffusion-limited crystal growth theory.

Related Experiment Videos

  • Developing a numerical model to simulate devitrification in human keratinocytes with glycerol.
  • Comparing simulations of cell warming with devitrification in H2O-NaCl-glycerol droplets.

    • Main Results:

    • Cell dehydration during warming affects intracellular ice formation, particularly for cells with low membrane transport activation energy.
    • The model predicts that critical warming rate increases with cooling rate, but this relationship can reverse due to dehydration during warming.
    • Intracellular nucleation rate is less sensitive to dehydration than crystal growth rate.

    Conclusions:

    • Cell dehydration is a critical factor influencing devitrification during cryopreservation warming.
    • The developed model provides insights into optimizing freeze-thaw protocols by considering water transport and dehydration effects.
    • Further research using this model can enhance cell survival rates in cryopreservation.