Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 28, 2026

Upright Imaging of Drosophila Embryos
07:34

Upright Imaging of Drosophila Embryos

Published on: September 14, 2010

Expression pattern of DMAP-85 during Drosophila embryonic development.

V Cambiazo1, M González, R B Maccioni

  • 1Laboratorio de Biología Celular y Molecular, Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425, Santiago, Chile. vcambiaz@uec.inta.uchile.cl

Mechanisms of Development
|October 2, 2001
PubMed
Summary

Drosophila Microtubule-Associated Protein 85 (DMAP-85) is crucial for microtubule organization during embryonic development. This study maps DMAP-85 distribution, revealing its role in stabilizing microtubules in the ventral nerve cord.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Infection and associated risk factors in the immediate postoperative period of pediatric liver transplantation: a study of 176 transplants.

Clinical transplantation·1998
Same author

Peripheral blood progenitor cells for autologous transplant in children.

Bone marrow transplantation·1998
Same author

Immunophenotypic characterization of plasma cells from monoclonal gammopathy of undetermined significance patients. Implications for the differential diagnosis between MGUS and multiple myeloma.

The American journal of pathology·1998
Same author

Unusual presentation of tuberculous rheumatism (Poncet's disease) with oral ulcers and tuberculid.

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America·1998
Same author

[Snake bite accidents in children in Costa Rica: epidemiology and determination of risk factors in the development of abscess and necrosis].

Toxicon : official journal of the International Society on Toxinology·1998
Same author

The innervation pattern of the radial nerve at the elbow and in the forearm.

Journal of hand surgery (Edinburgh, Scotland)·1998

Area of Science:

  • Cell Biology
  • Developmental Biology
  • Molecular Biology

Background:

  • Microtubule-associated proteins (MAPs) are essential regulators of cytoskeletal organization and integrity.
  • DMAP-85, a Drosophila MAP, has been previously identified for its role in promoting tubulin polymerization in vitro.

Purpose of the Study:

  • To investigate the embryonic distribution and microtubule association patterns of DMAP-85.
  • To understand the functional significance of DMAP-85 during Drosophila embryogenesis.

Main Methods:

  • Immunoblotting to determine DMAP-85 abundance across embryonic stages.
  • Immunofluorescence microscopy to visualize DMAP-85 localization and its association with microtubules.

Main Results:

More Related Videos

Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster
10:10

Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster

Published on: September 20, 2014

A Protocol for Immunohistochemistry and RNA In-situ Distribution within Early Drosophila Embryo
14:34

A Protocol for Immunohistochemistry and RNA In-situ Distribution within Early Drosophila Embryo

Published on: May 6, 2022

Related Experiment Videos

Last Updated: May 28, 2026

Upright Imaging of Drosophila Embryos
07:34

Upright Imaging of Drosophila Embryos

Published on: September 14, 2010

Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster
10:10

Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster

Published on: September 20, 2014

A Protocol for Immunohistochemistry and RNA In-situ Distribution within Early Drosophila Embryo
14:34

A Protocol for Immunohistochemistry and RNA In-situ Distribution within Early Drosophila Embryo

Published on: May 6, 2022

  • DMAP-85 expression is detected throughout embryogenesis, peaking in stages 6-9.
  • DMAP-85 associates with stable microtubules during cellularization and interphase arrays post-gastrulation.
  • At later stages, DMAP-85 localizes to axonal microtubules in the ventral nerve cord.

Conclusions:

  • DMAP-85 is dynamically associated with microtubules during Drosophila embryonic development.
  • The findings suggest DMAP-85 is vital for the stabilization and organization of cytoplasmic microtubules, particularly in the developing nervous system.