Quantitative fractography by digital image processing: NIH Image macro tools for stereo pair analysis and 3-D reconstruction
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Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)
Published on: November 1, 2017
1Laboratório de Análise de Imagens de Materiais (LAIMat), Departamento de Materiais e Tecnologia, Faculdade de Engenharia de Guaratinguetá, Universidade Estadual Paulista, 12.516-410, Guaratinguetá, SP, Brazil. rhein@feg.unesp.br
Abstract:
A set of NIH Image macro programs was developed to make qualitative and quantitative analyses from digital stereo pictures produced by scanning electron microscopes. These tools were designed for image alignment, anaglyph representation, animation, reconstruction of true elevation surfaces, reconstruction of elevation profiles, true-scale elevation mapping and, for the quantitative approach, surface area and roughness calculations. Limitations on time processing, scanning techniques and programming concepts are also discussed.

