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Quantitative amino acid analysis using a Beckman system gold HPLC 126AA analyzer.
Y Qu1, R H Slocum, J Fu
1H.A. Chapman Institute of Medical Genetics, 5300 East Skelly Drive, Tulsa, OK 74135, USA. yong_qu@nymc.edu
Summary
This study establishes an affordable amino acid (AA) analysis program using a Beckman 126AA Analyzer and Pickering reagents. The method provides accurate AA quantitation for diagnosing and monitoring metabolic disorders like phenylketonuria (PKU).
Area of Science:
- Clinical Chemistry
- Analytical Biochemistry
- Metabolic Disorders
Background:
- The widely used Beckman 6300/7300 analyzer for amino acid (AA) analysis is no longer available.
- This necessitates the development of alternative, affordable methods for AA quantitation.
Purpose of the Study:
- To establish an affordable amino acid analysis program using a Beckman System Gold HPLC 126AA analyzer.
- To develop and validate quantitative AA analysis methods for clinical diagnosis and monitoring.
Main Methods:
- Utilized a Beckman System Gold HPLC 126AA analyzer with Pickering Laboratories reagents.
- Developed two quantitative AA analysis programs: an 18-min short program for seven AAs (plasma, dried blood spots) and a 118-min long program for 40 AAs (plasma, urine).
- Employed Lithium eluents and varied column temperatures (32-72°C) for optimal separation.
Main Results:
- DBS specimen results showed good agreement with CDC certified values.
- Plasma and urine sample results correlated well with the discontinued Beckman 6300 analyzer (r = 0.9076–0.999).
Conclusions:
- Amino acid quantitation using the Beckman 126AA Analyzer and Pickering reagents is effective for clinical diagnosis.
- This method is valuable for monitoring patients with aminoacidopathies, such as phenylketonuria (PKU) and homocystinuria.