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Updated: Jul 19, 2026

Chromatin Immunoprecipitation from Human Embryonic Stem Cells
Published on: July 22, 2008
Establishment of Polycomb silencing requires a transient interaction between PC and ESC
1Department of Zoology, University of Geneva, CH-1211 Geneva, Switzerland.
Abstract:
Two distinct types of Polycomb complexes have been identified in flies and in vertebrates, one containing ESC and one containing PC. Using LexA fusions, we show that PC and ESC can establish silencing of a reporter gene but that each requires the presence of the other. In early embryonic extracts, we find PC transiently associated with ESC in a complex that includes EZ, PHO, PH, GAGA, and RPD3 but not PSC. In older embryos, PC is found in a complex including PH, PSC, GAGA, and RPD3, whereas ESC is in a separate complex including EZ, PHO, and RPD3.
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