Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Linking the Rb and polycomb pathways.

A Dahiya1, S Wong, S Gonzalo

  • 1Division of Molecular Oncology, Washington University School of Medicine, St. Louis, MO 63110, USA.

Molecular Cell
|October 5, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

UNITY: A low-field magnetic resonance neuroimaging initiative to characterize neurodevelopment in low and middle-income settings.

Developmental cognitive neuroscience·2024
Same author

Mental nerve shielding from possible injury during mandibular surgical procedures: technical note.

International journal of oral and maxillofacial surgery·2023
Same author

The way to a man's heart is through his stomach? A unique case report of transient constrictive pericarditis secondary to infarction of herniated omentum following bariatric surgery.

European heart journal. Case reports·2022
Same author

Considerations for Pregnant Dental and Health Care Workers amid COVID-19.

JDR clinical and translational research·2020
Same author

Human amygdala functional network development: A cross-sectional study from 3 months to 5 years of age.

Developmental cognitive neuroscience·2018
Same author

Mapping White Matter Microstructure in the One Month Human Brain.

Scientific reports·2017
Same journal

Genome-wide rotational and translational phasing of nucleosomes with human transcription factors.

Molecular cell·2026
Same journal

Spliceosomal proofreading factors safeguard 3' splice-site fidelity and prevent proteotoxicity and inflammation.

Molecular cell·2026
Same journal

Cytosolic EZH2-IMPDH2 complexes regulate melanoma progression and metastasis via GTP.

Molecular cell·2026
Same journal

A bacterial reverse transcriptase: Protein-templated DNA synthesis fuels antiviral immunity.

Molecular cell·2026
Same journal

Tweezing apart ribosome heterogeneity.

Molecular cell·2026
Same journal

An NADPH safety valve: De novo lipogenesis buffers biguanide-induced reductive stress.

Molecular cell·2026
See all related articles

Polycomb group (PcG) proteins and Rb form a repressor complex that halts cell division. This complex links cell cycle arrest to embryonic pattern formation, revealing a novel role for PcG proteins in cell proliferation.

Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Cell Cycle Regulation

Background:

  • Polycomb group (PcG) proteins are crucial for Hox gene repression during embryonic development.
  • PcG proteins also regulate cell proliferation independently of their role in Hox gene patterning.
  • The retinoblastoma (Rb) protein is a key regulator of the cell cycle.

Purpose of the Study:

  • To investigate the interaction between Rb and PcG proteins.
  • To determine the role of the Rb-PcG complex in cell cycle regulation.
  • To elucidate the link between cell cycle arrest and embryonic pattern formation.

Main Methods:

  • Co-immunoprecipitation assays to detect protein-protein interactions.
  • Immunofluorescence microscopy to assess protein colocalization.

Related Experiment Videos

  • Cell cycle analysis to evaluate the effect on mitosis.

    • Main Results:

    • Rb associates with PcG proteins to form a repressor complex.
    • This Rb-PcG complex inhibits cell entry into mitosis.
    • Rb is essential for PcG complex association with nuclear targets.
    • Rb and PcG proteins colocalize within the nucleus.

    Conclusions:

    • The Rb-PcG complex plays a significant role in cell cycle arrest.
    • This interaction provides a mechanism linking cell cycle control to differentiation and embryonic patterning.
    • PcG proteins have a dual function in development: Hox gene regulation and cell proliferation control.