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Updated: Oct 4, 2026
Measurement of Nitric Oxide Production in Mouse Tracheal Epithelial Cell Cultures Differentiated at the Air Liquid Interface
Published on: July 3, 2026
Effect of nitric oxide on proliferation of human retina pigment epithelial cells
1Baskent University, School of Medicine, Department of Ophthalmology, Ankara, Turkey. gurselyilmaz@hotmail.com
Purpose:
To investigate the effects of exogenous and endogenous nitric oxide (NO) on the proliferation of human retina pigment epithelial (RPE) cells.
Methods:
We stimulated cultured human RPE cells with 3-morphosydnonimine (SIN-1) to analyse the effect of exogenous NO. Incubation with a cytokine cocktail (interleukin 1-beta + interferon gamma + tumour necrosis factor alpha) plus lipopolysaccharide (LPS) induced cells to synthesise NO endogenously. The cultures were then incubated for 48 h, after which the cells were stained with crystal violet and absorbance at 550 nm was measured spectrophotometrically.
Results:
SIN-1 inhibited human RPE cell proliferation, while haemoglobin, an NO inhibitor, almost completely blocked the inhibitory effect. On the other hand, treatment with the cytokine cocktail plus LPS did not inhibit RPE cell proliferation.
Conclusion:
These findings confirm that exogenous NO inhibits human RPE cell proliferation, while endogenous NO has no such blocking effect.
