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A Strategy to Identify Compounds that Affect Cell Growth and Survival in Cultured Mammalian Cells at Low-to-Moderate Throughput
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A Strategy to Identify Compounds that Affect Cell Growth and Survival in Cultured Mammalian Cells at Low-to-Moderate Throughput

Published on: September 22, 2019

Cell culture test system for express analysis of cytotoxic and growth-stimulating effects of bioactive compounds

V G Chernikov1, S M Terekhov, T B Krokhina

  • 1Medical Genetic Research Center, Russian Academy of Medical Sciences; Research Center of Biomedical Technologies, Moscow.

Bulletin of Experimental Biology and Medicine
|October 5, 2001
PubMed

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Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media