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GC-MS determination of gamma-hydroxybutyric acid (GHB) in blood
1Commonwealth of Massachusetts, Slate Police Crime Laboratory, Forensic Toxicology Unit, 59 Horsepond Road, Sudbury, MA 01776, USA.
Forensic Science International
|October 6, 2001
Abstract:
A sensitive and specific gas chromatography-mass spectrometer (GC-MS) method using selective ion monitoring (SIM) has been developed for the quantification of gamma-hydroxybutyric acid (GHB) in blood. This method uses liquid-liquid extraction and disilyl-derivatization, without conversion to the gamma-butyrolactone (GBL), followed by GC-MS analysis using GHB-d6 as the internal standard. The method was linear from 0.1 to 20 mg/dl, and the limit of quantification using 50 microl of blood was determined to be 0.1 mg/dl.