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Updated: Sep 26, 2026
High-resolution Tandem Mass Spectrometry for Studying Chemical Constituents of Gynura bicolor DC
Published on: February 2, 2024
An integrated FIDI-MS and UPLC-Q-TOF/MS strategy for rapid functional screening and chemical characterization of
Minglin Xu1, Ziyue Ma1, Jianbin Liu1
1State Key Laboratory of Component-based Chinese Medicine, Haihe Laboratory of Modern Chinese Medicine, Tianjin Key Laboratory of TCM Chemistry and Analysis, Instrumental Analysis and Research Center, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, PR China.
Abstract:
A novel integrated strategy combining field-induced droplet ionization mass spectrometry (FIDI-MS) with ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) was developed for the rapid screening and identification of antioxidant constituents in herbal extracts. This approach enables real-time assessment of antioxidant activity within microdroplets and comprehensive compositional characterization of complex matrices. Applied to Polygonum multiflorum extract (PME), the method successfully identified known antioxidants such as 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside (TSG) and revealed additional bioactive phytochemicals. Quantitative FIDI-MS dose-response profiling determined the antioxidant potency of key compounds, with trans-TSG exhibiting superior activity (IC50 = 13.33 ± 1.06 μM) compared to its cis-isomer and emodin. By integrating functional screening, reliable chemical profiling, and targeted quantification, the strategy enabled comprehensive analysis of bioactive constituents in complex herbal samples. Furthermore, the utility of FIDI-MS, when coupled with chromatographic separation and high-resolution mass detection, is underscored as a complementary approach for functional and chemical interrogation of bioactive compounds in botanical matrices.