[Analysis of several genes using primer extension preamplification based on individual cell]
1Reproductive Medicine Center, Changhai Hospital, the Second Military Medical University, Shanghai, 200433 P.R. China. sf2102518@newpalm.com
Objective:
To analyze several genes at the same time in one individual cell using primer extension preamplification (PEP).
Methods:
The whole genome of individual cell was preamplified by PEP using 10 base random oligonucleotides. The aliquots(5 mu liter) from PEP were used to amplify SRY, ZP(3), RhCE, RhD and F VIII genes by nested PCR.
Results:
The five genes could be amplified following PEP-PCR. Lymphocyte of four genotypes were diagnosed by PEP-PCR; correct diagnoses were obtained in 95.6%.
Conclusion:
At least five genes can be analyzed by PEP-PCR based on one individual cell in this laboratory. This method could be used in preimplantation genetic diagnosis and noninvasive prenatal diagnosis.
