Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Valpha gene replacement in a TCRalpha knock-in mouse.

R Golub1, C Y Huang, O Kanagawa

  • 1Department of Immunology, University of Toronto, Toronto, Canada.

European Journal of Immunology
|October 10, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[A case of toxic shock syndrome secondary to severe bone marrow suppression induced by azathioprine in a patient with autoimmune hepatitis].

Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology·2026
Same author

[Epidemic characteristics and trend analysis of major injuries deaths among children and adolescents in Jiangsu Province from 2012 to 2021].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2024
Same author

HEIDI: an experiment-management platform enabling high-throughput fragment and compound screening.

Acta crystallographica. Section D, Structural biology·2024
Same author

Application of machine learning techniques in the diagnostic approach of PTSD using MRI neuroimaging data: A systematic review.

Heliyon·2024
Same author

[Investigation of familial tendency of endometriosis].

Zhonghua fu chan ke za zhi·2023
Same author

Associations and relative risks of pulmonary hypertension and lung diseases in individuals with methamphetamine use disorder.

Pulmonology·2023
Same journal

Fast Generation of F(Ab')<sub>2</sub> Fragments From Human IgG Using Fc-Fused IgG-Degrading Enzyme.

European journal of immunology·2026
Same journal

Generation of Regulatory T Cells Against Islet Neoantigen.

European journal of immunology·2026
Same journal

Complement Inhibition in the Clinic: Are We Doing Enough to Protect Patients From Infection?

European journal of immunology·2026
Same journal

Special Issue: yEFIS 3rd Symposium.

European journal of immunology·2026
Same journal

CRISPR/Cas9-Mediated Gene Knockout Reveals a Nonredundant Role for p16<sup>INK4A</sup> in Controlling TCR-Dependent and Independent CD8 T Cell Expansion.

European journal of immunology·2026
Same journal

Induction of Humoral and Cellular Immunity After SARS-CoV-2 JN.1 Vaccination in Individuals With and Without Prior Infection.

European journal of immunology·2026
See all related articles

T-cell receptor (TCR) alpha gene replacement was observed in mice, generating functional TCR alpha chain transcripts. This discovery suggests V-gene replacement can diversify the T cell repertoire and the immune system.

Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • The T cell receptor (TCR) is crucial for adaptive immunity.
  • V(D)J recombination is the primary mechanism for generating TCR diversity.
  • The potential for V-gene replacement in the TCR alpha locus was previously unexplored.

Purpose of the Study:

  • To investigate the occurrence and function of V-gene replacement in the TCR alpha locus.
  • To determine if V-gene replacement can generate functional TCR alpha chain transcripts.
  • To assess the potential of V-gene replacement in shaping the peripheral T cell repertoire.

Main Methods:

  • Utilized a TCR alpha chain knock-in mouse model.
  • Analyzed splenic T cells for functional TCR alpha chain transcripts.

Related Experiment Videos

  • Investigated V-gene replacement events at the Valpha-embedded heptamer site.

    • Main Results:

    • Demonstrated that V-gene replacement operates within the TCR alpha locus.
    • Identified functional TCR alpha chain transcripts resulting from Valpha-gene replacement.
    • Confirmed Valpha-gene replacement occurs at the Valpha-embedded heptamer site.

    Conclusions:

    • TCR alpha V-gene replacement is a viable mechanism for generating TCR diversity.
    • This process can contribute to shaping the peripheral T cell repertoire.
    • The conserved heptamer suggests V-gene replacement is a fundamental immune diversification mechanism shared by B and T cells.