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[The study of expression and distribution of PCNA in hypertrophic scar tissue]
1Department of Plastic Surgery, Union Hospital of Tongji Medical University, Wuhan 430022, PR China.
Objective:
To analyze the expression and the distribution of proliferation cell nuclear antigen (PCNA) in hypertrophic scar for identifying its certain characteristics during the fibroblast proliferation.
Methods:
Bioptic samples were taken form 12 cases of hypertrophic scar, 8 cases of matured scars and 20 cases of controlled normal skin. Each sample was immunohistochemically stained and examined with monoclonal antibody[PCNA(pc10)] technique. The level of PCNA expression was decided for evaluation of the fibroblast proliferation.
Results:
The level of PCNA in the hypertrophic scar(45.4 +/- 12.3) was significantly higher than mature scar(17.4 +/- 6.2), also normal skin(13.5 +/- 4.1, P < 0.01). But, there was no significant deference between the matured scar and the normal skin. The PCNA intracellular particles may sometimes be bound in the keratinocytes and the fibroblasts of some connective tissue.
Conclusion:
The fibroblast proliferation in the hypertrophic scar has kept a high level. During the process of hypertrophic scar formation it may play an important role.