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Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting
Published on: June 30, 2017
Human adipose tissue endothelial cells promote preadipocyte proliferation
L J Hutley1, A C Herington, W Shurety
1Department of Diabetes and Endocrinology, University of Queensland, Princess Alexandra Hospital, Woolloongabba, Queensland 4102, Australia 4001.
American Journal of Physiology. Endocrinology and Metabolism
|October 12, 2001
Summary
Human endothelial cells from adipose tissue secrete factors that promote preadipocyte proliferation. This suggests a crucial role for vascularization in adipose tissue development and function.
Area of Science:
- Cell Biology
- Endocrinology
- Vascular Biology
Background:
- Adipogenesis, the process of fat cell formation, requires extensive vascularization for adipose tissue to function as an energy store and endocrine organ.
- Endothelial cells form the inner lining of blood vessels and play a critical role in regulating tissue development and function.
Purpose of the Study:
- To investigate the role of endothelial cell-derived factors in promoting the proliferation of human preadipocytes.
- To determine if adipose tissue endothelial cells secrete soluble factors that influence preadipocyte growth.
Main Methods:
- Isolation of microvascular endothelial cells and preadipocytes from human omental and subcutaneous adipose tissue biopsies.
- Utilized collagenase digestion, immunoselection, and differential trypsinization for cell isolation.
- Cultured endothelial cells and collected conditioned medium to assess its effect on preadipocyte proliferation.
Main Results:
- Conditioned medium from microvascular endothelial cells significantly promoted preadipocyte proliferation (P < 0.001).
- The conditioned medium also promoted the proliferation of other cell types, to a lesser extent.
- No significant depot-specific differences were observed in the mitogenic capacity of endothelial cell medium or preadipocyte response.
Conclusions:
- Adipose tissue endothelial cells secrete soluble factors that stimulate preadipocyte proliferation.
- These findings highlight the importance of endothelial cells in regulating adipose tissue expansion and function.
- Endothelial cell-derived factors represent potential targets for modulating adipose tissue development.
