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Engineering Skeletal Muscle Tissues from Murine Myoblast Progenitor Cells and Application of Electrical Stimulation
Published on: March 19, 2013
Manufacturing, Cell Regulation, and Coculture Strategies for Vascularization and Neural Innervation of Skeletal
Zhihui Wang1, Xiaoyu He1, Shuangying Zhong2
1School of Medicine, Shanghai University, Shanghai, China.
Advanced Healthcare Materials
|August 14, 2026
Summary
Skeletal muscle tissue engineering (SMTE) faces challenges in replicating native muscle complexity. An integrated approach combining advanced fabrication, cellular regulation, and multicellular coordination is crucial for functional muscle reconstruction.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Tissue Engineering
Background:
- Skeletal muscle tissue engineering (SMTE) aims to restore function after volumetric muscle loss (VML).
- Current SMTE approaches struggle to replicate native muscle's complex anisotropic architecture, vascular networks, and neural structures.
- Achieving functional skeletal muscle requires overcoming significant fabrication, cellular, and coordination challenges.
Purpose of the Study:
- To propose an integrated framework for functional skeletal muscle reconstruction.
- To review advancements in structural fabrication, cellular regulation, and multicellular coordination for SMTE.
- To highlight the necessity of combining multiple strategies for successful muscle tissue engineering.
Main Methods:
- Review of hybrid fabrication strategies beyond single manufacturing methods.
- Emphasis on physical and chemical cues for cellular regulation (myogenesis, vascularization, innervation).
- Discussion of multicellular coculture systems for functional remodeling.
Main Results:
- Hybrid fabrication strategies are superior to single methods for replicating multiscale muscle structures.
- Multimodal cellular stimulation synergistically enhances myogenic differentiation, vascularization, and innervation.
- Coculture systems are essential for functional remodeling and clinical translation.
Conclusions:
- Functional skeletal muscle reconstruction necessitates an integrated framework combining advanced hybrid manufacturing, controlled cellular regulation, and robust multicellular coculture.
- Convergence of these strategies is key to reproducible construction of vascularized and innervated muscle constructs.
- Addressing clinical translation and regulatory challenges is vital for SMTE advancement.
