Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Calelectrin in human blood cells.

T C Südhof1, C W Zimmermann, J H Walker

  • 1Abteilung Neurochemie, Max-Planck-Institut für biophysikalische Chemie, Postfach 2841, 3400 Göttingen/Federal Republic of Germany.

European Journal of Cell Biology
|May 1, 1983
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cases of the Epidemic Cholera.

Edinburgh medical and surgical journal·2018
Same author

Developmental plasticity shapes synaptic phenotypes of autism-associated neuroligin-3 mutations in the calyx of Held.

Molecular psychiatry·2016
Same author

Conditional ablation of neuroligin-1 in CA1 pyramidal neurons blocks LTP by a cell-autonomous NMDA receptor-independent mechanism.

Molecular psychiatry·2016
Same author

Compositional and sensory differences of products of sweet-cream and whey buttermilk produced by microfiltration, diafiltration, and supercritical CO2.

Journal of dairy science·2015
Same author

Conditional neuroligin-2 knockout in adult medial prefrontal cortex links chronic changes in synaptic inhibition to cognitive impairments.

Molecular psychiatry·2015
Same author

Pathogenic mechanism of an autism-associated neuroligin mutation involves altered AMPA-receptor trafficking.

Molecular psychiatry·2015
Same journal

Epigenetic regulation of non-apoptotic regulated cell death.

European journal of cell biology·2026
Same journal

Corrigendum to "Insulin receptor-inspired soluble insulin binder" [Eur. J. Cell Biol. 102 (2023) 151293].

European journal of cell biology·2026
Same journal

Fucosylation in cancer and its role in anti-tumor immunity.

European journal of cell biology·2026
Same journal

Ethanol stress-induced vacuole unlobing is mediated via downregulation of phosphatidylinositol-3,5-bisphosphate and Atg18 phospho-regulation.

European journal of cell biology·2026
Same journal

Letter to the Editor: TGF-β drives pulmonary fibrosis through dual dysregulation of TNF pathway transcription and splicing.

European journal of cell biology·2026
Same journal

A new photochromic sterol controls TRPC3 activity by actuating a hub of regulatory lipid coordination.

European journal of cell biology·2026
See all related articles

Calelectrin, a calcium-binding protein, is found in human white blood cells, not platelets or red blood cells. This protein, conserved across species, plays a role in calcium-triggered cellular processes.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Biochemistry

Background:

  • Calelectrin is a novel calcium-binding protein identified in fish nerve terminals.
  • It exhibits membrane binding, self-aggregation, and calcium-dependent membrane aggregation.
  • Its presence and function in human cells were previously unknown.

Purpose of the Study:

  • To investigate the presence and localization of calelectrin in human blood cells.
  • To compare human calelectrin with its fish counterpart.
  • To explore the functional implications of calelectrin expression in human cells.

Main Methods:

  • Immunofluorescence microscopy was used to detect calelectrin in human blood cells.
  • Immune blotting and SDS-polyacrylamide gel electrophoresis analyzed protein fractions.

Related Experiment Videos

  • Fractionation of human blood cells (mononuclear cells, granulocytes, erythrocytes, thrombocytes) was performed.

    • Main Results:

    • Calelectrin was detected in all human leukocytes, including mononuclear cells, but absent in platelets and erythrocytes.
    • Immunofluorescence revealed a diffuse cytoplasmic localization, not associated with membranes or cytoskeleton.
    • Immune blotting confirmed calelectrin presence in granulocytes and mononuclear cells, with a similar molecular weight (34-35 kD) to Torpedo calelectrin.
    • Human calelectrin demonstrated calcium-dependent membrane binding.

    Conclusions:

    • Calelectrin is evolutionarily conserved between fish and humans.
    • Its expression in both neural and mesenchymal cells suggests a significant functional role.
    • The absence of calelectrin in platelets suggests it is not essential for exocytosis but may participate in other calcium-triggered events.