[Electrochemical sensor based on photopolymeric membranes for determining urea]
A V Rebriev1, S P Ivashkevich, N F Starodub
1Palladin Institute of Biochemistry of NAS of Ukraine, Kyiv.
Ukrains'Kyi Biokhimichnyi Zhurnal (1999 )
|October 16, 2001
Summary
A novel electrochemical sensor using ion selective field effect transistors (ISFETs) and a photocurable membrane was developed for rapid urea determination. This sensor offers a practical solution for medical diagnostics, providing accurate results within minutes.
Area of Science:
- Electrochemistry
- Biosensor Technology
- Materials Science
Context:
- Urea determination is crucial in medical diagnostics.
- Existing methods may be time-consuming or complex.
- Need for rapid, reliable urea sensing.
Purpose:
- To develop a novel electrochemical enzymatic sensor for urea detection.
- To immobilize urease onto an ISFET using a photocurable membrane.
- To evaluate the sensor's performance, including linear range, analysis time, and stability.
Summary:
- A new sensor utilizes ion selective field effect transistors (ISFETs) and a photocurable membrane for urea determination.
- Urease was immobilized on the ISFET gate using a photocurable composition.
- The sensor exhibits a linear response from 0.05-20 mM, with analysis times of 5-10 minutes and a working duration of at least 40 days.
Impact:
- The developed ISFET-based sensor shows promise for rapid blood urea analysis.
- The membrane preparation method is compatible with integrated semiconductor biosensor fabrication.
- Facilitates faster and potentially more accessible diagnostic testing.
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