Solid-phase extraction and GC-MS analysis of THC-COOH method optimized for a high-throughput forensic drug-testing...

Area of Science:

• Forensic Toxicology
• Analytical Chemistry
• Pharmacology

Background:

• Accurate confirmation of delta9-tetrahydrocannabinol (THC) in numerous urine samples is crucial.
• Existing methods may be time-consuming or generate significant waste.

Purpose of the Study:

• To develop an efficient extraction, derivatization, and GC-MS method for THC confirmation in urine.
• To improve laboratory workflow and safety in forensic toxicology.

Main Methods:

• Utilized anion-exchange solid-phase extraction with positive pressure manifold.
• Employed rapid derivatization with pentafluoropropionic anhydride/pentafluoropropanol.
• Analyzed samples using Gas Chromatography-Mass Spectrometry (GC-MS).

Main Results:

• Achieved average recoveries of 95% and a limit of detection of 0.875 ng/mL.
• Demonstrated linearity up to 900 ng/mL THC-COOH using a deuterated internal standard.
• Validated the method on over 1000 human urine samples, confirming excellent agreement with prior results and identifying an improved qualifier ion (m/z 622).

Conclusions:

• The developed method provides a highly efficient and accurate approach for THC confirmation in urine.
• Significant reductions in processing time, waste, and personnel exposure were achieved.
• The method enhances laboratory safety and throughput for forensic drug testing.