Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Identification of flatfish (Pleuronectiforme) species using DNA-based techniques.

C G Sotelo1, P Calo-Mata, M J Chapela

  • 1Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Cientificas, Eduardo Cabello 6, 36208 Vigo, Spain. carmen@iim.csic.es

Journal of Agricultural and Food Chemistry
|October 16, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of fish oil in Iberian sow diets on fatty acid, oxylipins and immune traits of colostrum and milk, and suckling piglets' growth performance.

Animal : an international journal of animal bioscience·2025
Same author

Six weeks of a concurrent training therapy improves endothelial function and arterial stiffness in hypertensive adults with minimum non-responders.

Hipertension y riesgo vascular·2024
Same author

Comparison of sediment bioavailable methods to assess the potential risk of metal(loid)s for river ecosystems.

Ecotoxicology and environmental safety·2024
Same author

The impact of loneliness and social isolation on the benefits of an exercise program with hospitalised older adults.

The journal of nutrition, health & aging·2024
Same author

Reduced Handgrip Strength Is Associated with 1 Year-Mortality in Brazilian Frail Nonagenarians and Centenarians.

The Journal of frailty & aging·2024
Same author

Erratum to: Tailored Prevention of Functional Decline through a Multicomponent Exercise Program in Hospitalized Oncogeriatric Patients: Study Protocol for a Randomized Clinical Trial.

The journal of nutrition, health & aging·2024
Same journal

Inclusion of <i>Limosilactobacillus fermentum</i> CECT5716 in Novel Fermented Caprine Milk: Technological and Nutritional Assessment.

Journal of agricultural and food chemistry·2026
Same journal

Lipidomic and Transcriptomic Insights into Hepatic Lipid Remodeling Induced by Peony Seed Oil.

Journal of agricultural and food chemistry·2026
Same journal

Evolution Process of Pseudo-lignin Derived from Lignocellulose.

Journal of agricultural and food chemistry·2026
Same journal

Distinct Chemical Profiles and Gut Microbiota Modulation of Ancha Tea Compared with Green and Black Teas from the Same Cultivar.

Journal of agricultural and food chemistry·2026
Same journal

Highly Stereoselective Synthesis of the Chiral Fungicide's Intermediate (<i>R</i>)-<i>N</i>-(2,6-Dimethylphenyl) Aminopropionic Acid Methyl Ester via an Esterase-Lipase Cascade.

Journal of agricultural and food chemistry·2026
Same journal

Modernizing Pesticide Risk Assessment: Technical Advancements as the Path toward Global Food Security.

Journal of agricultural and food chemistry·2026
See all related articles

DNA analysis using mitochondrial cytochrome b gene effectively identifies most flatfish species. A faster PCR-RFLP method differentiates 21 species, offering a cost-effective alternative to sequencing for flatfish identification.

Area of Science:

  • Marine Biology
  • Molecular Genetics
  • Fisheries Science

Background:

  • Accurate identification of flatfish species is crucial for fisheries management and conservation.
  • Traditional methods can be time-consuming and may lack precision.
  • DNA-based approaches offer a powerful tool for species identification.

Purpose of the Study:

  • To develop and evaluate a DNA-based method for identifying flatfish species.
  • To assess the utility of mitochondrial cytochrome b gene sequences for flatfish differentiation.
  • To establish a rapid and cost-effective molecular technique for flatfish identification.

Main Methods:

  • Polymerase chain reaction (PCR) amplification of a 464 bp fragment from the mitochondrial cytochrome b gene.
  • Sequence analysis, genetic distance calculations, and phylogenetic tree construction for 24 flatfish species.

Related Experiment Videos

  • Selection of restriction enzymes for polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis.

    • Main Results:

    • The mitochondrial cytochrome b gene fragment was highly polymorphic, enabling differentiation of most species.
    • Phylogenetic analysis grouped species into distinct clades, with two exceptions (Limanda spp. and Solea spp.).
    • PCR-RFLP successfully differentiated 21 out of 24 flatfish species, providing specific restriction profiles.

    Conclusions:

    • DNA sequencing of the mitochondrial cytochrome b gene is effective for flatfish species identification.
    • PCR-RFLP offers a faster, less expensive, and reliable alternative for identifying multiple flatfish species.
    • This molecular methodology is suitable for application with commercial flatfish samples.