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Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro
09:16

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro

Published on: November 26, 2018

Essential role of ICAM-1 in mediating monocyte adhesion to aortic endothelial cells

C G Kevil1, R P Patel, D C Bullard

  • 1Department of Genomics and Pathobiology, University of Alabama at Birmingham, 35294-0019, USA.

American Journal of Physiology. Cell Physiology
|October 16, 2001
PubMed

Insights

Intercellular adhesion molecule-1 (ICAM-1) is crucial for monocyte adhesion to endothelial cells in vascular diseases. Genetic studies show ICAM-1 deficiency completely inhibits monocyte binding, highlighting its critical role.

Area of Science:

  • Vascular Biology
  • Immunology
  • Cellular Adhesion

Background:

  • Monocyte-endothelial cell interactions are key in vascular diseases like atherosclerosis.
  • Intercellular adhesion molecule-1 (ICAM-1) is a suspected mediator of monocyte adhesion, but its precise role is debated.

Purpose of the Study:

  • To genetically determine the contribution of ICAM-1 in mediating monocyte adhesion to mouse aortic endothelial cells (MAEC).

Main Methods:

  • Utilized a genetic approach comparing wild-type and ICAM-1 knockout (ICAM-1(-/-)) MAEC.
  • Assessed monocyte adhesion following stimulation with oxidized low-density lipoprotein and tumor necrosis factor-alpha.

Main Results:

  • Oxidized low-density lipoprotein significantly increased monocyte adhesion to wild-type MAEC.
  • ICAM-1(-/-) MAEC showed complete inhibition of monocyte binding when treated with oxidized low-density lipoprotein.
  • Tumor necrosis factor-alpha also induced monocyte adhesion to wild-type MAEC, with significant binding only at higher doses in ICAM-1(-/-) MAEC.

Conclusions:

  • ICAM-1 plays a critical role in mediating monocyte-endothelial cell interactions.
  • These interactions are vital in response to stimuli involved in inflammatory vascular diseases.

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