Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA exhibits multi-stranded binding recognition on glass microarrays.

S J Shi1, A Scheffer, E Bjeldanes

  • 1Incyte Genomics, Microarray Division, 6519 Dumbarton Circle, Fremont, CA 94555, USA.

Nucleic Acids Research
|October 16, 2001
PubMed
Summary

Researchers observed unexpected multi-stranded DNA structures on microarrays, challenging classical base pairing. These findings suggest novel DNA interactions on surfaces, potentially modeling biological processes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Regional variability in the Acheulian to Middle Stone Age transition in southern Africa.

Scientific reports·2026
Same author

[Digital loop-mediated isothermal amplification detection technology and its application progress].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2021
Same author

[Progress in research of allergen detection methods].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2021
Same author

[Preliminary study of the antibody level in confirmed patients with COVID-19 after discharge].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2020
Same author

[Research advances on the molecular mechanisms of vascular permeability in sepsis].

Zhonghua shao shang za zhi = Zhonghua shaoshang zazhi = Chinese journal of burns·2020
Same author

[Transmission electron microscopic observation on gonad of <i>Oncomelania hupensis</i> offspring bred in Weishan Lake areas].

Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control·2020

Area of Science:

  • Molecular Biology
  • Biophysics
  • Materials Science

Background:

  • Classical Watson-Crick base pairing governs DNA hybridization.
  • DNA microarrays are widely used for genetic analysis.
  • Unusual DNA structures can arise under specific experimental conditions.

Purpose of the Study:

  • To investigate unexpected hybridization patterns observed on glass DNA microarrays.
  • To characterize the formation and properties of multi-stranded DNA structures on surfaces.
  • To explore potential biological relevance of these observed DNA interactions.

Main Methods:

  • Utilized DNA microarrays with varying sequences (purine and pyrimidine bases).
  • Performed hybridization experiments with single-stranded (ssDNA) and double-stranded (duplex) DNA probes.

Related Experiment Videos

  • Analyzed binding interactions, including sequence identity and strand orientation preference.

    • Main Results:

    • Observed hybridization of double-stranded DNA to ssDNA array elements and vice versa.
    • Demonstrated the formation of multi-stranded DNA structures on microarray surfaces.
    • Identified a binding preference for duplexes with a 3'-homologous strand near their ends.

    Conclusions:

    • Classical base pairing models do not fully explain observed DNA hybridization on microarrays.
    • Multi-stranded DNA structures form on cationic solid surfaces under specific conditions.
    • These interactions may serve as models for biological processes involving complex DNA structures.