Related Experiment Video

Updated: Aug 7, 2026

Isolation of Murine Coronary Vascular Smooth Muscle Cells
08:24

Isolation of Murine Coronary Vascular Smooth Muscle Cells

Published on: May 30, 2016

[Antisense oligonucleotides inhibit c-myc and PCNA expression in the vascular smooth muscle cells]

J Bian1, B Zhang, S Chen

  • 1Department of Vascular Surgery, Renji Hospital, Shanghai Second Medical University, Shanghai 200001.

Zhonghua Yi Xue Za Zhi
|October 17, 2001
PubMed
Abstract

Insights

Antisense oligonucleotides targeting c-myc and PCNA effectively inhibit vascular smooth muscle cell proliferation, offering a potential strategy for preventing vascular restenosis.

Area of Science:

  • Molecular biology
  • Cardiovascular research
  • Gene therapy

Context:

  • Vascular restenosis, characterized by smooth muscle cell hyperplasia, is a significant complication after vascular interventions.
  • Understanding the molecular mechanisms driving vascular smooth muscle cell (VSMC) proliferation is crucial for developing effective treatments.

Purpose:

  • To investigate the efficacy of antisense oligonucleotides targeting c-myc and PCNA in inhibiting VSMC proliferation.
  • To evaluate the potential of antisense technology for preventing and treating vascular restenosis.

Summary:

  • Antisense oligonucleotides (ASOs) specifically designed against c-myc and PCNA were synthesized and delivered to cultured VSMCs.
  • RT-PCR analysis demonstrated that antisense c-myc and PCNA at 10 µmol/L significantly inhibited gene expression (P < 0.05).
  • Sense and mismatched oligonucleotides did not show significant inhibitory effects (P > 0.05), confirming the specificity of the antisense approach.

Impact:

  • The study validates antisense technology as a potent method for suppressing VSMC proliferation.
  • This finding holds promise for developing novel therapeutic strategies to combat vascular restenosis and improve patient outcomes.

More Related Videos

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro08:28

Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro

Published on: February 15, 2022

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...