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Related Experiment Videos

Establishing a reference system in clinical enzymology.

M Panteghini1, F Ceriotti, G Schumann

  • 1Laboratorio Analisi Chimico Cliniche 1, Azienda Ospedaliera Spedali Civili, Brescia, Italy. panteghi@bshosp.osp.unibs.it

Clinical Chemistry and Laboratory Medicine
|October 17, 2001
PubMed
Summary
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Standardizing enzyme measurements ensures comparable results across labs. The International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) developed a reference system using validated procedures and certified materials for accurate clinical enzymology.

Area of Science:

  • Clinical enzymology
  • Biochemical standardization
  • Laboratory medicine

Background:

  • Enzyme measurements require standardization for reliable comparison across different laboratories, kits, and instruments.
  • The International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) initiated a project to create a robust reference system in clinical enzymology.

Purpose of the Study:

  • To establish a comprehensive reference system for enzyme measurements.
  • To ensure comparability of enzyme concentration results in human samples.
  • To enable traceability of routine laboratory results to reference procedures.

Main Methods:

  • Development and refinement of reference procedures for key enzymes.
  • Certification of reference materials for alanine aminotransferase, creatine kinase, gamma-glutamyltransferase, and lactate dehydrogenase.

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  • Establishment of a network of reference laboratories for controlled execution of procedures.

    • Main Results:

    • Modified IFCC-recommended procedures optimized for 37°C with detailed operating instructions.
    • Re-certification of partially purified and stabilized enzyme materials.
    • Industry can now assign traceable values to commercial calibrators using certified materials.

    Conclusions:

    • The IFCC reference system provides a framework for accurate and comparable enzyme measurements.
    • Traceable calibrators enable clinical laboratories to achieve consistent results for human samples.
    • This standardization improves the reliability of clinical enzymology diagnostics.