Related Experiment Video
Updated: Jul 4, 2026

Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins
Published on: January 17, 2014
Unique temperature-sensitive defect in vaccinia virus morphogenesis maps to a single nucleotide substitution in the
P Szajner1, A S Weisberg, B Moss
1Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892-0445, USA.
Abstract:
Marker rescue experiments demonstrated that the genetic lesion of a previously isolated vaccinia virus temperature-sensitive mutant which forms multilayered envelope structures with lucent interiors and foci of viroplasm with dense centers mapped to the A30L open reading frame. A single base change, resulting in a nonconservative Ser-to-Phe substitution at residue 17, was associated with degradation of the A30L protein at elevated temperatures.
Related Concept Videos
Viral Mutations
Leaky Scanning
Mutations in Microorganisms
Point and Frameshift Mutations

