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Published on: June 8, 2012
Effects of tripcholorolide on inflammatory reaction of mouse alveolar macrophages in vitro
1Department of Critical Care Medicine, Zhong-Da Hospital of Southeast University, Nanjing 210009, China. haiboq@hotmail.com
Aim:
To observe the effects of tripcholorolide (T4) on inflammatory reaction of mouse alveolar macrophages.
Methods:
RT-PCR was used to investigate tumor necrosis factor alpha (TNF alpha), interleukin-1 beta (IL-1 beta), IL-6, IL-10, and inducible nitric-oxide synthase (iNOS) mRNA expression in alveolar macrophages after LPS 10 mg/L and T4 500 micrograms/L treatment. ELISA was used to detect TNF alpha, IL-1 beta, IL-6, and IL-10 protein expression. Nitrite was measured by Griess reaction.
Results:
TNF alpha, IL-1 beta, IL-6, IL-10, and nitrite increased in supernatants, when alveolar macrophages were stimulated by LPS 10 mg/L at 24 h. Both T4 500 micrograms/L and dexamethasone 100 mumol/L had inhibitory effects on the production of TNF alpha, IL-1 beta, IL-6, IL-10, and nitric oxide. The mRNA expression of TNF alpha, IL-6, IL-10, and iNOS increased at 5 h after LPS stimulation which was decreased on addition of T4 500 micrograms/L or dexamethasone 100 mumol/L. T4 had no effect on stability of LPS-induced mRNA expression in TNF alpha, IL-6, and IL-10.
Conclusion:
T4 had inhibitory effects on the expression of proinflammatory and antiinflammatory mediators.
