Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Dual function for U2AF(35) in AG-dependent pre-mRNA splicing.

S Guth1, T Ø Tange, E Kellenberger

  • 1Gene Expression Programme, European Molecular Biology Laboratory, 69117 Heidelberg, Germany.

Molecular and Cellular Biology
|October 18, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Successful simultaneous stenting of a pulmonary artery and vein in pulmonary vascular stenosis due to silicosis. Case report and literature review.

Frontiers in cardiovascular medicine·2023
Same author

Improved Metal Cation Optosensing Membranes through the Incorporation of Sulphated Polysaccharides.

Molecules (Basel, Switzerland)·2022
Same author

[Congenital Pulmonary Artery Stenoses as a Rare Cause of Pulmonary Hypertension].

Pneumologie (Stuttgart, Germany)·2021
Same author

Development of concomitant diseases in COVID-19 critically ill patients.

Revista espanola de anestesiologia y reanimacion·2020
Same author

Correlation of native T1 mapping with right ventricular function and pulmonary haemodynamics in patients with chronic thromboembolic pulmonary hypertension before and after balloon pulmonary angioplasty.

European radiology·2018
Same author

Effects of isoflavones on breast tissue and the thyroid hormone system in humans: a comprehensive safety evaluation.

Archives of toxicology·2018
Same journal

Aberrant Expression of miR-25-3p/EZH2 Is Involved in T Cell Activation in Aplastic Anemia.

Molecular and cellular biology·2026
Same journal

Characterization of the m<sup>6</sup>A Epitranscriptome in Fibroblast Senescence.

Molecular and cellular biology·2026
Same journal

Insights into FACT in Cancers with Targeted Therapeutic Implications.

Molecular and cellular biology·2026
Same journal

Human lncRNA, hLinfRNA7 (IDO1-AS) Regulates Cytokine Expression, Tryptophan Catabolism, and Inflammatory Response in Macrophage.

Molecular and cellular biology·2026
Same journal

mTORC1-Dependent Regulation of the CCL24-CCR3 Axis Controls Granuloma Formation and Maintenance in Sarcoidosis.

Molecular and cellular biology·2026
Same journal

The Novel Compound SIC-19 Triggers CUL4B-Mediated Ubiquitination and Degradation of SIK2.

Molecular and cellular biology·2026
See all related articles

The splicing factor U2AF(35) plays a dual role in pre-mRNA splicing, stabilizing U2AF(65) binding and triggering spliceosome assembly. Its function is linked to AG-dependent splicing and the 3' splice site, independent of SR protein interactions.

Area of Science:

  • Molecular Biology
  • RNA Splicing Mechanisms

Background:

  • U2AF is crucial for U2 small nuclear RNP recruitment to pre-mRNAs in eukaryotes.
  • U2AF(65) binds polypyrimidine tracts, while U2AF(35) interacts with the 3' splice site AG.
  • U2AF(35) can stabilize U2AF(65) binding to weak polypyrimidine tracts and mediate interactions with SR proteins.

Purpose of the Study:

  • To investigate sequence elements governing U2AF(35) dependence in mouse immunoglobulin M (IgM) pre-mRNA splicing.
  • To elucidate the specific functions of U2AF(35) beyond stabilizing U2AF(65) binding.

Main Methods:

  • Oligo(dT)-cellulose chromatography to deplete nuclear extracts of U2AF.
  • Pre-mRNA splicing complementation assays using depleted nuclear extracts.
  • Analysis of sequence elements including polypyrimidine tract strength, 3' splice site, and exonic splicing enhancers (ESE).

Related Experiment Videos

Main Results:

  • IgM pre-mRNA is AG-dependent, and U2AF(35) dependence correlates with this AG dependence.
  • The first nucleotide of exon 2 is critical for U2AF(35) function.
  • RS domain-mediated interactions with SR proteins are dispensable for U2AF(35) activity in this context.

Conclusions:

  • U2AF(35) has a dual function in pre-mRNA splicing: stabilizing U2AF(65) binding and initiating spliceosome assembly events.
  • U2AF(35) interaction with the consensus 3' splice site is key to its function.
  • The findings reveal novel aspects of U2AF(35)'s role in splicing regulation.