Related Experiment Video
Updated: Jul 28, 2026

Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction
Published on: May 28, 2012
Structural analysis of the lipopolysaccharide from the nontypable Haemophilus influenzae strain SB 33
A D Cox1, H Masoud, P Thibault
1Institute for Biological Sciences, National Research Council, Ottawa, ON, Canada. Andrew.Cox@nrc.ca
Abstract:
The structure of the core region of the lipopolysaccharide (LPS) from the nontypable Haemophilus influenzae strain SB 33 was elucidated. The LPS was subjected to a variety of degradative procedures. The structures of the derived oligosaccharide products were established by monosaccharide and methylation analyses, NMR spectroscopy and mass spectrometry. These analyses revealed a series of related phosphocholine (PCho) containing structures differing in the number of hexose residues. The results pointed to each species containing a conserved phosphoethanolamine (PEtn) substituted heptose-containing trisaccharide inner-core moiety. The major LPS glycoforms were identified as 2-Hex, 3-Hex and 4-Hex species according to the number of hexose residues present.
Related Concept Videos
Outer Layers of the Cell Envelope
Surface Appendages of Archaea
Peptidoglycan Synthesis
Formation of Lipopolysaccharides
Determinants of Bacterial Pathogenicity and Virulence

