Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Plant histochemistry by correlation peak imaging.

A Metzler1, M Izquierdo, A Ziegler

  • 1Physikalisches Institut V, Universität Würzburg, Würzburg, Germany.

Proceedings of the National Academy of Sciences of the United States of America
|December 5, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The impact of lipid distribution, composition and mobility on xylem water refilling of the resurrection plant Myrothamnus flabellifolia.

The New phytologist·2021
Same author

A bridged loop gap resonator (BLGR) for small animal imaging by 1.5 T MRI systems.

The Review of scientific instruments·2020
Same author

Insights into possibilities for grouping and read-across for nanomaterials in EU chemicals legislation.

Nanotoxicology·2018
Same author

Minute Impurities Contribute Significantly to Olfactory Receptor Ligand Studies: Tales from Testing the Vibration Theory.

eNeuro·2017
Same author

Analysis of 2D NMR relaxation data using Chisholm approximations.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2017
Same author

A simple metal staining procedure for identification and visualization of single cells by LA-ICP-MS.

The Analyst·2017

This study used NMR imaging to map metabolites in castor bean seedlings, revealing distinct spatial patterns for sugars and amino acids within plant tissues. These findings highlight NMR

Area of Science:

  • Plant Physiology
  • Metabolomics
  • Biophysical Chemistry

Background:

  • Understanding plant metabolism is crucial for agriculture and biotechnology.
  • Noninvasive techniques are needed to study metabolite distribution in living plants.
  • Castor bean seedlings are a model system for studying plant development and biochemistry.

Purpose of the Study:

  • To investigate the spatial distribution of carbohydrates and amino acids in castor bean hypocotyls.
  • To demonstrate the potential of a new NMR correlation-peak imaging technique for plant metabolite analysis.
  • To explore metabolic heterogeneity in living plant tissues.

Main Methods:

  • Utilized a novel NMR correlation-peak imaging technique.
  • Performed noninvasive analysis of metabolite spatial distribution.

Related Experiment Videos

  • Focused on the hypocotyl of castor bean seedlings.

    • Main Results:

    • Identified high sucrose concentrations in phloem and cortex parenchyma, but low levels in pith parenchyma.
    • Observed lower glucose concentrations in cortex parenchyma compared to pith parenchyma.
    • Detected glutamine/glutamate in cortex parenchyma and vascular bundles, lysine/arginine in vascular bundles, and valine in cortex parenchyma.

    Conclusions:

    • Spectroscopic NMR imaging can noninvasively study plant physiology.
    • Demonstrated the potential for analyzing spatial metabolic heterogeneity in living plants.
    • Metabolite distribution patterns suggest complex physiological roles within plant tissues.